EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.11.30 | DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN + 2-oxoglutarate + O2 |
- |
Homo sapiens |
DESGLPQLTSYDAEV-(3S)-3-hydroxy-L-asparaginyl-APIQGSRNLLQGEELLRALDQVN + succinate + CO2 |
- |
? |
1.14.11.30 | DESGLPQLTSYDCEVNAPI + 2-oxoglutarate + O2 |
hypoxia-inducible factor-1alpha peptide 788-806. 9% of the activity obtained with the 35-amino-acid HIF-1alpha peptide DES35 (DESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRAL) |
Homo sapiens |
? |
- |
? |
1.14.11.30 | DESGLPQLTSYDCEVNAPIQGSR + 2-oxoglutarate + O2 |
hypoxia-inducible factor-1alpha peptide 788-810. 15% of the activity obtained with the 35-amino-acid HIF-1alpha peptide DES35 (DESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRAL) |
Homo sapiens |
? |
- |
? |
1.14.11.30 | DESGLPQLTSYDCEVNAPIQGSRNLLQ + 2-oxoglutarate + O2 |
hypoxia-inducible factor-1alpha peptide 788-814. 37% of the activity obtained with the 35-amino-acid HIF-1alpha peptide DES35 (DESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRAL) |
Homo sapiens |
? |
- |
? |
1.14.11.30 | DESGLPQLTSYDCEVNAPIQGSRNLLQGEEL + 2-oxoglutarate + O2 |
hypoxia-inducible factor-1alpha peptide 788-818. 26% of the activity obtained with the 35-amino-acid HIF-1alpha peptide DES35 (DESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRAL) |
Homo sapiens |
? |
- |
? |
1.14.11.30 | DESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRAL + 2-oxoglutarate + O2 |
hypoxia-inducible factor-1alpha peptide 788-822 |
Homo sapiens |
? |
- |
? |
1.14.11.30 | ESYLLPELTRYDCEVNVPVLGSSTLLQGGDLLRAL + 2-oxoglutarate + O2 |
hypoxia-inducible factor-2alpha peptide 832-857. 7% of the activity obtained with the 35-amino-acid HIF-1alpha peptide DES35 (DESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRAL) |
Homo sapiens |
? |
- |
? |
1.14.11.30 | hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 |
- |
Homo sapiens |
hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2 |
- |
? |
1.14.11.30 | hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 |
activity of the hypoxia-inducible factor (HIF) complex is controlled by oxygen-dependent hydroxylation of prolyl and asparaginyl residues. Hydroxylation of specific prolyl residues by 2-oxoglutarate-dependent oxygenases mediates ubiquitinylation and proteasomal destruction of HIF-alpha. Hydroxylation of an asparagine residue (ASn803) in the C-terminal transactivation domain of HIF-alpha abrogates interaction with p300, preventing transcriptional activation |
Homo sapiens |
hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2 |
- |
? |
1.14.11.30 | hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 |
the activity of hypoxia-inducible transcription factor HIF, an alphabeta heterodimer that has an essential role in adaptation to low oxygen availability, is regulated by two oxygen-dependent hydroxylation events. Hydroxylation of specific proline residues by HIF prolyl 4-hydroxylases targets the HIF-alpha subunit for proteasomal destruction, whereas hydroxylation of an asparagine in the C-terminal transactivation domain prevents its interaction with the transcriptional coactivator p300 |
Homo sapiens |
hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2 |
- |
? |