EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.13.12.24 | coelenterazine + O2 |
- |
Aequorea victoria |
coelenteramide + CO2 + hnu |
- |
? |
1.13.12.24 | h-coelenterazine + O2 |
- |
Aequorea victoria |
h-coelenteramide + CO2 + hnu |
- |
? |
1.13.12.24 | more |
aequorin exhibits an absorption near 454 nm. The native chromophore is Renilla luciferin or a nearly identical derivative. The binding of calcium ion to the photoprotein brings about a conformational change in the protein which results in the chromophore reacting with a protein side chain hydroperoxide to yield a protein bound luciferin hydroperoxide intermediate. This intermediate then decomposes to CO2 and an electronic excited state of the corresponding protein-oxyluciferin monoanion complex which leads to light emission |
Aequorea victoria |
? |
- |
? |
1.13.12.24 | more |
no substrates: bisdeoxycoelenterazine, C6-methoxycoelenterazine, e-coelenterazine |
Aequorea victoria |
? |
- |
? |
1.13.12.24 | more |
poor substrates: n-coelenterazine, bis-coelenterazine, methoxy-coelenterazine, e-coelenterazine |
Aequorea victoria |
? |
- |
? |
1.13.12.24 | more |
the luminescence peak of aequorin is found at 472 nm. In presence of high concentrations of green fluorescent protein, the peak shifts to 509 nm |
Aequorea victoria |
? |
- |
? |
1.13.12.24 | [aequorin] 1,2-dioxetan-3-one |
reaction in presence of Ca2+ |
Aequorea victoria |
[aequorin] coelenteramide + CO2 + hnu |
- |
? |
1.13.12.24 | [apoaequorin] + coelenterazine + O2 |
- |
Aequorea victoria |
[aequorin] 1,2-dioxetan-3-one |
- |
? |
1.13.12.24 | [apoaequorin] + coelenterazine + O2 |
- |
Aequorea victoria |
[apoaequorin] + coelenteramide + CO2 + hnu |
- |
? |
1.13.12.24 | [apoaequorin] + coelenterazine + O2 + 3 Ca2+ |
- |
Aequorea victoria |
[excited state blue fluorescent protein] + CO2 |
- |
? |