EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.1.1.335 | 2-acetamido-2-deoxy-alpha-D-glucuronic acid + NAD+ + L-glutamate |
activity of enzyme His6-WbpB is detected only in presence of transaminase WbpE, and vice versa |
Pseudomonas aeruginosa |
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid + NADH + 2-ketoglutarate |
product of the coupled reaction of enzyme WbpB and transaminase WbpE |
? |
1.1.1.335 | more |
the reaction mechanism is sequential, enzyme does not require 2-ketoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine |
Bordetella pertussis |
? |
- |
? |
1.1.1.335 | more |
the reaction mechanism is sequential, enzyme does not require 2-oxoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine |
Chromobacterium violaceum |
? |
- |
? |
1.1.1.335 | UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+ |
- |
Bordetella pertussis |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ |
- |
? |
1.1.1.335 | UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+ |
- |
Chromobacterium violaceum |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ |
- |
? |
1.1.1.335 | UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+ |
isolated enzyme EWbpB in presence of UDP-2-acetamido-2-deoxy-alpha-D-glucuronate and NAD+ does not show enzymic activity. Upon the addition of WbpE and L-glutamate to the reaction, complete turnover of the starting material and the formation of UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid are observed. No turnover is observed in the presence of UDP-N-acetyl-D-glucosamine or UDP-UDP-N-acetyl-D-galactosamine, and only minimal turnover is observed when UDP-D-glucuronic acid is used as the nucleotide sugar substrate. Enzyme WbpB prefers the glucopyranose configuration of the sugar as well as the presence of both the carboxylate at the C'' carbon and the acetylated amine at the C'' position. WbpE is specific for L-glutamate as the amine donor. Presence of 2-ketoglutarate is required as oxidant for NAD+ recycling |
Pseudomonas aeruginosa |
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ |
- |
? |
1.1.1.335 | UDP-alpha-D-glucuronate + NAD+ |
- |
Bordetella pertussis |
UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+ |
- |
? |
1.1.1.335 | UDP-alpha-D-glucuronate + NAD+ |
- |
Chromobacterium violaceum |
UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+ |
- |
? |
1.1.1.335 | UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O |
- |
Porphyromonas gingivalis |
UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+ |
- |
? |
1.1.1.335 | UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O |
- |
Porphyromonas gingivalis DSM 20709 |
UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+ |
- |
? |