EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
6.3.3.6 | ATP + (2S,5S)-5-carboxymethylproline |
- |
Pectobacterium carotovorum |
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate |
- |
? |
6.3.3.6 | ATP + (2S,5S)-5-carboxymethylproline |
- |
Streptomyces cattleya |
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate |
- |
? |
6.3.3.6 | ATP + (2S,6R)-2,6-dimethyl-t-carboxymethylproline |
- |
Pectobacterium carotovorum |
AMP + diphosphate + (3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate |
- |
? |
6.3.3.6 | ATP + (2S,6S)-2,6-dimethyl-t-carboxymethylproline |
- |
Pectobacterium carotovorum |
AMP + diphosphate + (2S,3S,5S,6S)-3,6-dimethylcabapenam-3-carboxylate |
- |
? |
6.3.3.6 | ATP + (3R,6R)-2,6-dimethyl-t-carboxymethylproline |
- |
Pectobacterium carotovorum |
AMP + diphosphate + (2R,3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate |
- |
? |
6.3.3.6 | ATP + (3R,6S)-2,6-dimethyl-t-carboxymethylproline |
- |
Pectobacterium carotovorum |
AMP + diphosphate + (2R,3S,5S,6S)-3,6-dimethylcabapenam-3-carboxylate |
- |
? |
6.3.3.6 | ATP + (3S,6R)-2,6-dimethyl-t-carboxymethylproline |
- |
Pectobacterium carotovorum |
AMP + diphosphate + (2S,3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate |
- |
? |
6.3.3.6 | ATP + (2S,5S)-5-carboxymethylproline |
a mechanism is proposed where the rate-limiting step is beta-lactam ring formation coupled to a protein conformational change. The role of K443 throughout the reaction is undercored |
Pectobacterium carotovorum |
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate |
- |
? |
6.3.3.6 | ATP + (2S,5S)-5-carboxymethylproline |
catalytic Tyr-Glu dyad is demonstrated by site-directed mutagenesis and kinetic experiments that compare the wild-type enzymes to their respective mutant proteins using pHยrate profiles, 32P-incorporation experiments, solvent isotope effects, proton inventory, and viscosity variation |
Pectobacterium carotovorum |
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate |
- |
? |
6.3.3.6 | ATP + (2R,5R)-5-carboxymethylproline |
low activity, kcat/Km is 2% compared to the value for (2S,5S)-5-carboxymethylproline |
Pectobacterium carotovorum |
? |
- |
? |