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EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
5.6.2.2
negatively supercoiled pBR322 DNA
ATP-dependent positive supercoiling activity
Acidianus infernus
?
-
?
5.6.2.2
catenated kDNA + ATP + H2O
-
African swine fever virus
decatenated kDNA + ADP + phosphate
-
?
5.6.2.2
DNA + ATP + H2O
-
African swine fever virus
DNA + ADP + phosphate
-
?
5.6.2.2
more
the enzyme cannot supercoil relaxed DNA
African swine fever virus
?
-
?
5.6.2.2
supercoiled pBR322 plasmid DNA + ATP + H2O
-
African swine fever virus
relaxed pBR322 plasmid DNA + ADP + phosphate
-
ir
5.6.2.2
more
substrate specificity
Arabidopsis thaliana
?
-
?
5.6.2.2
supercoiled dsDNA
relaxation of supercoiled dsDNA, reaction mechanism
Arabidopsis thaliana
?
-
?
5.6.2.2
DNA
positively supercoils DNA, the enzyme requires ATP, which is bound and hydrolysed by a helicase-like domain
Archaeoglobus fulgidus
positively supercoiled DNA
-
?
5.6.2.2
negatively supercoiled pBR322 DNA
ATP-dependent positive supercoiling activity
Archaeoglobus fulgidus
?
-
?
5.6.2.2
ATP + relaxed pUC18 DNA
enzyme that binds and hydrolyzes only one ATP undergoes nucleotide-induced N-gate closure and supercoils DNA
Bacillus subtilis
ADP + phosphate + supercoiled pUC18 DNA
-
?
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