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Results 1 - 10 of 84 > >>
EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
Abz-TEGEARGSVI-Dap(2,4-dinitrophenyl)-KK + H2O
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?
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aggrecan + H2O
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?
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aggrecan + H2O
100 microg/ml
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-
?
aggrecan + H2O
2-8 microg, assay at pH 7.2, 37°C, reaction stopped by addition of 21 mM EDTA
?
-
?
aggrecan + H2O
2mg/ml, assay at 37°C, 16 h
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?
aggrecan + H2O
40 microg
?
-
?
aggrecan + H2O
ADAMTS-4 and ADAMTS-5 are the proteases responsible for the endogenous aggrecanase activity observed in IL-1beta stimulated bovine cartilage
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?
aggrecan + H2O
cleavage at the Glu373-Ala374 site of aggrecan is measured
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-
?
aggrecan + H2O
non-conservative mutation at P2' position of the bovine aggrecan (R375L) does not result in an observable difference in ADAMTS-4 susceptibility. Mutations in recombinant bovine aggrecan at Ser377 to Thr or Asn do not result in any significant difference in ADAMTS-4 cleavage. The S377Q mutation results in a significant reduction of cleavage by ADAMTS-4. The S377Q mutant is susceptible to cleavage by the C-terminally truncated ADAMTS-4. The S377A mutant is cleaved similarly to wild-type aggrecan
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aggrecan + H2O
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fragments of aggrecan
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?
Results 1 - 10 of 84 > >>