EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.1.21.3 | duplex DNA + ATP |
- |
Citrobacter freundii |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate |
- |
? |
3.1.21.3 | duplex DNA + ATP |
overview of recognition sequences |
Citrobacter freundii |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate |
- |
? |
3.1.21.3 | DNA + H2O |
EcoR124I couples ATP hydrolysis to bidirectional DNA translocation |
Escherichia coli |
? |
- |
? |
3.1.21.3 | DNA + H2O |
the enzyme must overcome a similar slow step before translocation reaches a steady state |
Escherichia coli |
? |
- |
? |
3.1.21.3 | DNA + H2O |
HsdR subunit can produce only a single cleavage of the phosphodiester backbone of the DNA but can cooperate with another HsdR subunit to produce full DNA cleavage, producing DNA with overhanging ends of single-stranded DNA. On a single-site plasmid, cleavage requires the association of HsdR, from solution, to the cleavage complex in order to produce double-strand cleavage |
Escherichia coli |
? |
- |
? |
3.1.21.3 | duplex DNA + ATP |
- |
Escherichia coli |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate |
- |
? |
3.1.21.3 | duplex DNA + ATP |
overview of recognition sequences |
Escherichia coli |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate |
- |
? |
3.1.21.3 | duplex DNA + ATP |
the wild type enzyme EcoK cleaves circular DNA. Only one endonuclease molecule is required per cleavage event. Cleavage of linear DNA may require a second endonuclease molecule |
Escherichia coli |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate |
- |
? |
3.1.21.3 | duplex DNA + ATP |
the enzyme is both a restriction endonuclease and a modification methylase. Hemi-methylated DNA is the preferred substrate for methylation |
Escherichia coli |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate |
- |
? |
3.1.21.3 | duplex DNA + ATP |
the site of restriction cleavage is random, occuring between 1 and 5 kb from the recognition site. The modification methylase acts directly at the recognition sequence |
Escherichia coli |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate |
- |
? |