EC Number   |
Substrates |
Organism |
Products |
Reversibility  |
|---|
   2.1.1.267 | more |
substrate specificity, overview |
Nemophila menziesii |
? |
- |
- |
| 2.1.1.267 | more |
substrate specificity study using a number of potential substrates including anthocyanidins, anthocyanins, flavonols, flavones, flavan-3-ols, and phenolic acid as substrates based on optimized conditions, overview. Enzyme PsAOMT uses anthocyanins as methoxyl accepters, and acts to methylate the 3'-hydroxyl group of the B-ring with high affinity and efficiency. Pelargonidin 3-O-glucoside is the only tested anthocyanin compound that is not a substrate for PsAOMT. With delphinidin 3-O-glucoside, a sequential methylation occurs at the 3'- and 5'-hydroxyl group on the B-ring. In comparison with the substrates delphinidin 3-O-glucoside, quercetin 3-O-rutinoside, and quercetin, PsAOMT has a higher affinity for cyanidin 3-O-glucoside and cyanidin 3,5-O-diglucoside. Cyanidin-derived anthocyanins are high-affinity substrates for PsAOMT. No activity with the antocyanin pelargonidin 3-O-glucoside and the cyanidin delphinin. The enzyme also shows no activity with kaempferol 3-O-glucoside, kaempferol, apigenin, naringenin, epicatechin, and caffeic acid |
Paeonia suffruticosa |
? |
- |
- |
| 2.1.1.267 | more |
substrate specificity study using a number of potential substrates including anthocyanidins, anthocyanins, flavonols, flavones, flavan-3-ols, and phenolic acid as substrates based on optimized conditions, overview. Enzyme PtAOMT uses anthocyanins as methoxyl accepters, and acts to methylate the 3'-hydroxyl group of the B-ring with high affinity and efficiency. Pelargonidin 3-O-glucoside is the only tested anthocyanin compound that is not a substrate for PtAOMT. With delphinidin 3-O-glucoside, a sequential methylation occurs at the 3'- and 5'-hydroxyl group on the B-ring. In comparison with the substrates delphinidin 3-O-glucoside, quercetin 3-O-rutinoside, and quercetin, PtAOMT has a higher affinity for cyanidin 3-O-glucoside and cyanidin 3,5-O-diglucoside. Cyanidin-derived anthocyanins are high-affinity substrates for PtAOMT. No activity with the antocyanin pelargonidin 3-O-glucoside and the cyanidin delphinin. The enzyme also shows no activity with kaempferol 3-O-glucoside, kaempferol, apigenin, naringenin, epicatechin, and caffeic acid |
Paeonia tenuifolia |
? |
- |
- |
| 2.1.1.267 | more |
the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity |
Citrus depressa |
? |
- |
- |
| 2.1.1.267 | more |
the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity. Isozyme CdFOMT5 exhibits O-methyltransferase activity for quercetin, naringenin, (-)-epicatechin, and equol using S-adenosyl-L-methionine (SAM) as a methyl donor. The recombinant CdFOMT5 CdFOMT5 can catalyze the O-methylation of at least three hydroxyl groups of quercetin, and di- or tri-O-methylated quercetin products are obtained by this enzymatic reaction. CdFMOT5 prefers flavonol (3-hydroxyflavone) to other flavonoid structures. Thus, substrate structure, especially the C-ring in flavonoids, may strongly affect the substrate preference, including regioselectivity, of CdFOMT5 |
Citrus depressa |
? |
- |
- |
| 2.1.1.267 | 2 S-adenosyl-L-methionine + myricetin |
the enzyme also catalyzes the methylation of luteolin, tricetin and caffeoyl-CoA. ROMT-15 exhibits similar kcat/Km values for the four substrates. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity |
Oryza sativa |
2 S-adenosyl-L-homocysteine + syringetin |
syringetin is the 3',5'-dimethyl ether of myricetin |
? |
| 2.1.1.267 | 2 S-adenosyl-L-methionine + myricetin |
the enzyme also catalyzes the methylation of luteolin, tricetin and caffeoyl-CoA. ROMT-17 prefers tricetin. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity |
Oryza sativa |
2 S-adenosyl-L-homocysteine + syringetin |
syringetin is the 3',5'-dimethyl ether of myricetin |
? |
| 2.1.1.267 | 4 S-adenosyl-L-methionine + quercetin |
whole-cell biocatalysis using CdFOMT5 expressed in Escherichia coli cells is performed using quercetin as a substrate, and 3,3',5,7-tetramethylated quercetin is obtained as the final product |
Citrus depressa |
4 S-adenosyl-L-homocysteine + 3,3',5,7-tetramethoxyquercetin |
- |
? |
| 2.1.1.267 | eriodictyol + S-adenosyl-L-methionine |
11% of the activity with quercetin |
Solanum lycopersicum |
homoeriodictyol + S-adenosyl-L-homocysteine |
- |
? |
| 2.1.1.267 | laricitrin + S-adenosyl-L-methionine |
114% of the activity with quercetin |
Solanum lycopersicum |
syringetin + S-adenosyl-L-homocysteine |
- |
? |
