EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.4.1.B4 | L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+ |
- |
Campylobacter jejuni |
carboxynorspermidine + NADP+ + H2O |
- |
? |
1.4.1.B4 | carboxynorspermidine + NAD+ + H2O |
NADH is a much poorer cofactor than NADPH |
Vibrio alginolyticus |
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+ |
- |
ir |
1.4.1.B4 | carboxynorspermidine + NADP+ + H2O |
i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed |
Vibrio alginolyticus |
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+ |
i.e. (2S)-2-amino-4-oxobutanoate |
ir |
1.4.1.B4 | carboxyspermidine + NADP+ + H2O |
i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane |
Vibrio alginolyticus |
L-aspartic 4-semialdehyde + putrescine + NADPH + H+ |
- |
ir |
1.4.1.B4 | more |
no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde |
Vibrio alginolyticus |
? |
- |
? |
1.4.1.B4 | carboxynorspermidine + NAD+ + H2O |
NADH is a much poorer cofactor than NADPH |
Vibrio alginolyticus ATCC 17749 |
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+ |
- |
ir |
1.4.1.B4 | carboxynorspermidine + NADP+ + H2O |
i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed |
Vibrio alginolyticus ATCC 17749 |
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+ |
i.e. (2S)-2-amino-4-oxobutanoate |
ir |
1.4.1.B4 | carboxyspermidine + NADP+ + H2O |
i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane |
Vibrio alginolyticus ATCC 17749 |
L-aspartic 4-semialdehyde + putrescine + NADPH + H+ |
- |
ir |
1.4.1.B4 | more |
no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde |
Vibrio alginolyticus ATCC 17749 |
? |
- |
? |