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EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
1.3.1.14
5-fluorodihydroorotate + NAD+
more active substrate for reverse reaction than orotate
Faecalicatena orotica
5-fluoroorotate + NADH + H+
-
r
1.3.1.14
more
-
Faecalicatena orotica
?
-
?
1.3.1.14
more
NAD+ binding domain in the beta subunit of enzyme, Cys-135 plays a catalytic role and Lys-48 is important for orienting the substrate in the active site and is able to interact with FMN
Lactococcus lactis
?
-
?
1.3.1.14
more
not: uracil, cytosine, 5-methylcytosine, thymine as substrates
Faecalicatena orotica
?
-
?
1.3.1.14
more
3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c
Faecalicatena orotica
?
-
?
1.3.1.14
more
O2, fumarate, and NADP+ do not serve as electron acceptors
Lactococcus lactis
?
-
?
1.3.1.14
(S)-dihydroorotate + NAD+
-
Lactococcus lactis
orotate + NADH + H+
-
r
1.3.1.14
(S)-dihydroorotate + NAD+
-
Faecalicatena orotica
orotate + NADH + H+
-
?
1.3.1.14
(S)-dihydroorotate + NAD+
-
Faecalicatena orotica
orotate + NADH + H+
-
r
1.3.1.14
(S)-dihydroorotate + NAD+
-
Lactococcus lactis
orotate + NADH + H+
-
?
Results
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