EC Number   |
Substrates |
Organism |
Products  |
Reversibility |
|---|
    1.14.99.54 | regenerated amorphous cellulose + 3-methylcatechol + O2 |
- |
Thermothelomyces thermophilus |
3-methyl-o-benzoquinone + H2O |
- |
? |
| 1.14.99.54 | cellooligosaccharide + pyrogallol + O2 |
- |
Achaetomiella virescens |
? |
- |
? |
| 1.14.99.54 | microcrystalline cellulose + AH2 + O2 |
- |
Thermobifida fusca |
? |
enzyme catalyzes release of a mixture of soluble sugars comprising reduced and oxidized cellooligosaccharides. The degree of polymerization of the released oligosaccharides ranges from 3 to 5 for the reduced products and from 2 to 5 for the oxidized products |
? |
| 1.14.99.54 | more |
The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone |
Thermothelomyces thermophilus |
? |
- |
? |
| 1.14.99.54 | more |
enzyme is a family AA13 protein acting on alpha-linked glycosidic bonds |
Aspergillus oryzae |
? |
- |
? |
| 1.14.99.54 | more |
for isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give the highest formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid |
Thermothelomyces thermophilus |
? |
- |
? |
| 1.14.99.54 | more |
isoform CelS2 produces C1-oxidized cellooligosaccharides only |
Streptomyces coelicolor |
? |
- |
? |
| 1.14.99.54 | more |
mechanism may follow one electron reduction of PMO-Cu(II) to PMO-Cu(I) by the cellobiose dehydrogenase heme domain followed by oxygen binding and internal electron transfer to form a copper superoxo intermediate. Hydrogen atom abstraction by the copper superoxo at the 1-position of an internal carbohydrate then takes place, generating a copper hydroperoxo intermediate and a substrate radical. The second electron from cellobiose dehydrogenase then facilitates O-O bond cleavage releasing water and generating a copper oxo radical that couples with the substrate radical, thereby hydroxylating the polysaccharide. The additional oxygen atom destabilizes the glycosidic bond leading to elimination of the adjacent glucan and formation of a sugar lactone or ketoaldose |
Neurospora crassa |
? |
- |
? |
| 1.14.99.54 | more |
mechanism may follow one electron reduction of PMO-Cu(II) to PMO-Cu(I) by the cellobiose dehydrogenase heme domain followed by oxygen binding and internal electron transfer to form a copper superoxo intermediate. Hydrogen atom abstraction by the copper superoxo at the 4-position of an internal carbohydrate then takes place, generating a copper hydroperoxo intermediate and a substrate radical. The second electron from cellobiose dehydrogenase then facilitates O-O bond cleavage releasing water and generating a copper oxo radical that couples with the substrate radical, thereby hydroxylating the polysaccharide. The additional oxygen atom destabilizes the glycosidic bond leading to elimination of the adjacent glucan and formation of a sugar lactone or ketoaldose |
Neurospora crassa |
? |
- |
? |
| 1.14.99.54 | more |
no substrate: beta-(1->3, 1->4)-glucan. For isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give the highest formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid |
Thermothelomyces thermophilus |
? |
- |
? |
