EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.19.39 | palmitoleoate + AH2 + O2 |
via intermediate DELTA9,12 hexadecadienoate |
Acanthamoeba castellanii |
9,12,15-hexadecatrienoate + A + H2O |
the product is n-1 polyunsaturated |
? |
1.14.19.39 | more |
key enzyme in adaptation to low temperature, sequential changes in fatty acid composition on lowering environmental temperature, overview |
Acanthamoeba castellanii |
? |
- |
? |
1.14.19.39 | more |
substrate specificity, the recombinant enzyme catalyzes also the DELTA15-desaturation of fatty acids, and is capable of producing very unusual n-1 polyunsaturated products, overview |
Acanthamoeba castellanii |
? |
- |
? |
1.14.19.39 | more |
H6Cf0807 is an acetylenase, that produces a trans DELTA12 double bond, while H6Cf0745 is a classical FAD2 desaturase. The Cantharellus formosus FAD2 homologue H6Cf0745 expression results in the production of only 16:2 DELTA9,12 and 18:2 DELTA9,12 in recombinant yeast cells supplemented with 18:2 DELTA9c,12c. Expression ofH6Cf0807 results in the production of crepenynic acid, 18:1 DELTA9c,12a. No C16 acetylenic fatty acid is detected. Supplementation with 18:2 DELTA9c,12t, alpha-linolenic acid, 18:3 DELTA9,12,15, or gamma-linolenic acid, 18:3 DELTA6,9,12, does not result in the production of other fatty acids, overview |
Cantharellus formosus |
? |
- |
? |
1.14.19.39 | more |
the enzyme is able to desaturate C16 and C18 fatty acids. When recombinantly expressed in yeast cells, the enzyme can also catalyze DELTA15 desaturation. The recombinant enzyme can also produce C14:2DELTA9,12, C15:2DELTA9,12, C17:2DELTA9,12, and C18:4DELTA6,9,12,15 when C14:1DELTA9, C15:1DELTA9, C17:1DELTA9, and C18:3DELTA6,9,12 substrates are available in yeast cells, product mass spectrometry analysis |
Caenorhabditis elegans |
? |
- |
? |
1.14.19.39 | phosphatidylcholine + AH2 + O2 |
- |
Acanthamoeba castellanii |
? |
- |
? |
1.14.19.39 | phosphatidylcholine + reduced cytochrome b5 + O2 |
with NADH |
Physcomitrium patens |
? + cytochrome b5 + 2 H2O |
- |
? |
1.14.19.39 | linoleate + AH2 + O2 |
- |
Caenorhabditis elegans |
crepenynate + A + 2 H2O |
- |
? |
1.14.19.39 | linoleate + reduced acceptor + O2 |
- |
Crepis alpina |
crepenynate + acceptor + H2O |
- |
? |
1.14.19.39 | linoleate + reduced acceptor + O2 |
first committed step in the biosynthesis of fatty acid-derived acetylenic secondary natural products, e.g. matricaria esters |
Crepis alpina |
crepenynate + acceptor + H2O |
- |
? |