EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
    1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin |
CYP101D1, CYP101D2 |
Novosphingobium aromaticivorans |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + reduced ferredoxin + O2 |
- |
Novosphingobium aromaticivorans |
(+)-exo-5-hydroxycamphor + oxidized ferredoxin + H2O |
- |
? |
| 1.14.15.1 | (+)-camphor + reduced ferredoxin + O2 |
- |
Novosphingobium aromaticivorans |
(+)-exo-5-hydroxycamphor + oxidized ferredoxin + H2O |
99% 5-exo-hydroxycamphor by wild-type enzyme and 92% by Y96A mutant |
? |
| 1.14.15.1 | (+)-camphor + reduced ferredoxin + O2 |
substrate of CYP101D1 and CYP101D2 |
Novosphingobium aromaticivorans |
(+)-exo-5-hydroxycamphor + oxidized ferredoxin + H2O |
- |
? |
| 1.14.15.1 | 2-adamantanone + O2 + reduced putidaredoxin |
CYP101D1 and CYP101D2 |
Novosphingobium aromaticivorans |
5-hydroxy-2-adamantanone + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | 3 2-methylpentane + 3 reduced ferreredoxin + O2 |
- |
Novosphingobium aromaticivorans |
2-methyl-pentan-2-ol + 2-methyl-pentan-3-ol + 2-methyl-pentan-4-ol + 3 oxidized ferredoxin |
52.5% 2-methyl-pentan-2-ol + 13% 2-methyl-pentan-3-ol, and 3% 2-methyl-pentan-4-ol for the wild-type enzyme, 5% + 12% + 30% for the mutant Y96A |
? |
| 1.14.15.1 | adamantane + reduced ferreredoxin + O2 |
- |
Novosphingobium aromaticivorans |
1-adamantol + 2-adamantol + oxidized ferredoxin + H2O |
98% 1-adamantol + 2% 2-adamantol for the wild-type enzyme, 97% + 3% for the mutant Y96A |
? |
| 1.14.15.1 | beta-ionone + O2 + reduced putidaredoxin |
CYP101C1 and CYP101B1. CYP101C1 oxidizes beta-ionone to 4-hydroxy-beta-ionone (75%) with one other, unidentified product. This latter compound is the major product of beta-ionone oxidation by CYP101B1 (90%) where 4-hydroxy-beta-ionone is the minor product (10%) |
Novosphingobium aromaticivorans |
4-hydroxy-beta-ionone + oxidized putidaredoxin + H2O |
- |
? |
| 1.14.15.1 | cyclooctane + reduced ferreredoxin + O2 |
- |
Novosphingobium aromaticivorans |
cyclooctanol + cyclooctanone + oxidized ferredoxin + H2O |
99% cyclooctanol + 1% cyclooctanone for the wild-type enzyme, 97% + 3% for the mutant Y96A |
? |
| 1.14.15.1 | hexane + reduced ferreredoxin + O2 |
- |
Novosphingobium aromaticivorans |
hexan-2-ol + hexan-3-ol + oxidized ferredoxin + H2O |
56% hexan-2-ol + 44% hexan-3-ol for the wild-type enzyme and mutant Y96A |
? |
