EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
    1.14.11.8 | N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 |
N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine |
Bos taurus |
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 |
- |
? |
| 1.14.11.8 | more |
determination of the (2S,3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine and N6,N6,N6-trimethyl-L-lysine is performed by ultraperformance liquid chromatographytandem mass spectrometry (UPLC/MS/MS) in positive ion electrospray mode |
Homo sapiens |
? |
- |
? |
| 1.14.11.8 | more |
NMR spectrosopic structure analysis of substrates and products |
Homo sapiens |
? |
- |
? |
| 1.14.11.8 | more |
NMR spectrosopic structure analysis of substrates and products, substrate specificity, overview. No or poor activity with dimethyllysine, methyllysine, lysine, D-trimethyllysine, 5-trimethylamino-1-aminopentane, trimethyllysine, 6-trimethylaminohexanoic acid, hexamethyllysine, N-acetyltrimethyllysine, trimethyldiaminobutyric acid, triethyllysine, symmetric and asymmetric dimethylarginines, mildronate, and gamma-butyrobetaine, structure-activity relationship study |
Homo sapiens |
? |
- |
? |
| 1.14.11.8 | N5,N5,N5-trimethyl-L-ornithine + 2-oxoglutarate + O2 |
- |
Homo sapiens |
(3S)-3-hydroxy-N5,N5,N5-trimethyl-L-ornithine + succinate + CO2 |
- |
? |
| 1.14.11.8 | N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 |
- |
Homo sapiens |
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 |
- |
? |
| 1.14.11.8 | N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 |
- |
Homo sapiens |
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 |
- |
? |
| 1.14.11.8 | N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 |
the enzymatic hydroxylation occurs at the C-3 site of (2S)-Nepsilon-trimethyllysine substrate. Comparative NMR spectroscopic studies (with two enantiopure synthetic standards that possess 3R and 3S stereochemistry) on the enzymatically produced (2S)-3-hydroxy-Nepsilon-trimethyllysine reveal that TMLH exclusively catalyzes the formation of (3S)-3-hydroxy-Nepsilon-trimethyl-L-lysine, not forming any (3R)-3-hydroxy-Nepsilon-trimethyl-L-lysine diastereoisomer |
Homo sapiens |
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 |
- |
? |
| 1.14.11.8 | N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 |
recognition sites that contribute to the enzymatic activity of the enzyme (TMLH): the Fe(II)-binding H242-D244-H389 residues, R391-R398 involved in 2-oxoglutarate-binding, and several residues (D231, N334, and the aromatic cage comprised of W221, Y217 and Y234) associated with binding of (2S)-Nepsilon-trimethyllysine |
Homo sapiens |
(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 |
- |
? |
| 1.14.11.8 | N6,N6-dimethyl-N6-ethyl-L-lysine + 2-oxoglutarate + O2 |
- |
Homo sapiens |
(3S)-3-hydroxy-N6,N6-dimethyl-N6-ethyl-L-lysine + succinate + CO2 |
- |
? |
