EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.4.22.14 | 2-(acetamido)ethylene 2'-pyridyl disulfide + H2O |
reactivity probe, analysis of temperature-dependence of reaction kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | 2-(acetoxy)ethylene 2'-pyridyl disulfide + H2O |
reactivity probe, analysis of temperature-dependence of reaction kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | 2-(N'-acetyl-D-phenylalanyl)hydroxyethylene 2'-pyridyl disulfide + H2O |
reactivity probe, analysis of temperature-dependence of reaction kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | 2-(N'-acetyl-D-phenylalanylamino)ethylene 2'-pyridyl disulfide + H2O |
reactivity probe, analysis of temperature-dependence of reaction kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | 2-(N'-acetyl-L-phenylalanyl)hydroxyethylene 2'-pyridyl disulfide + H2O |
reactivity probe, analysis of temperature-dependence of reaction kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | 2-(N'-acetyl-L-phenylalanylamino)ethylene 2'-pyridyl disulfide + H2O |
reactivity probe, analysis of temperature-dependence of reaction kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | ethyl 2-pyridyl disulfide + H2O |
reactivity probe, analysis of temperature-dependence of reaction kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | N-acetyl-L-Phe-Gly methyl ester + H2O |
stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | Insulin B-chain + H2O |
the enzyme cleaves most readily the carboxyl peptide bonds of those residues of the B-chain acylated by large hydrophobic residues - Val, Leu, or Phe, but not Tyr - and also the Arg22-Gly23 bond |
Actinidia chinensis |
? |
- |
? |
3.4.22.14 | Collagen type I + H2O |
the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), actinidin digests more than half of the collagen type I, but in acetate buffer (pH 4), the enzyme does not hydrolyze this substrate |
Actinidia deliciosa |
? |
- |
? |