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EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
3.4.22.14
butyloxycarbonyl-Phe-NH-CH2CN + H2O
-
Actinidia chinensis
?
-
?
3.4.22.14
casein + H2O
-
Actinidia deliciosa
?
-
?
3.4.22.14
casein + H2O
-
Actinidia chinensis
?
-
?
3.4.22.14
casein + H2O
efficiently hydrolized in acidic pHs
Actinidia deliciosa
?
-
?
3.4.22.14
casein + H2O
efficiently hydrolized in acidic pHs
Actinidia deliciosa cv. Hayward
?
-
?
3.4.22.14
Collagen + H2O
efficiently hydrolized at neutral or mild basic pH
Actinidia deliciosa
?
-
?
3.4.22.14
Collagen + H2O
efficiently hydrolized at neutral or mild basic pH
Actinidia deliciosa cv. Hayward
?
-
?
3.4.22.14
Collagen type I + H2O
actinidin can hydrolyze collagen types I and II at neutral and alkaline pH
Actinidia deliciosa
?
-
?
3.4.22.14
Collagen type I + H2O
the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), actinidin digests more than half of the collagen type I, but in acetate buffer (pH 4), the enzyme does not hydrolyze this substrate
Actinidia deliciosa
?
-
?
3.4.22.14
Collagen type I + H2O
actinidain cleaves between Gly1032 and Gly1033, limited hydrolysis by actinidain protease produces monomeric collagen, which consisted almost entirely of alpha1 and alpha2 chains
Actinidia deliciosa
?
-
?
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