EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.4.22.14 | Hemoglobin + H2O |
- |
Actinidia chinensis |
? |
- |
? |
| 3.4.22.14 | human occludin + H2O |
human occludin is fragmented, and putative Act d1 cleavage sites are identified in extracellular loops of human occludin |
Actinidia deliciosa |
? |
- |
? |
| 3.4.22.14 | Insulin B-chain + H2O |
the enzyme cleaves most readily the carboxyl peptide bonds of those residues of the B-chain acylated by large hydrophobic residues - Val, Leu, or Phe, but not Tyr - and also the Arg22-Gly23 bond |
Actinidia chinensis |
? |
- |
? |
| 3.4.22.14 | kiwellin + H2O |
in vitro treatment of purified kiwellin, an allergenic protein formerly isolated from green kiwi fruit, with the protease actinidin from green kiwi fruit originates KiTH, a 20 kDa protein with 100% identity with the C-terminal region of kiwellin, and kissper, a described pore-forming peptide |
Actinidia deliciosa |
KiTH + kissper |
- |
? |
| 3.4.22.14 | more |
mechanism |
Actinidia chinensis |
? |
- |
? |
| 3.4.22.14 | more |
the interplay of electrostatic and binding interactions determine the active center chemistry and catalytic activity |
Actinidia chinensis |
? |
- |
? |
| 3.4.22.14 | more |
actinidain, from the juice of kiwi fruit, has no detrimental effect on either the motility of Trichuris muris or the nematode cuticle |
Actinidia chinensis |
? |
- |
? |
| 3.4.22.14 | more |
actinidin compared with type II or IV collagenase isolated from intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90% |
Actinidia deliciosa |
? |
- |
? |
| 3.4.22.14 | more |
digestibility of kiwifruit allergens, actinidin (Act d 1) and thaumatin-like protein (Act d 2), is assessed using an in vitro digestion system that approximates physiological conditions with respect to the passage of food through the stomach into the duodenum: Act d 1 precipitates in simulated gastric fluid at pH 2 and digestion of the aggregated protein proceeds slowly. The residual precipitate redissolves completely in simulated duodenal fluid at pH 6.5 and is partially digested. Act d 1 and Act d 2 display nearly unchanged IgE binding abilities |
Actinidia deliciosa |
? |
- |
? |
| 3.4.22.14 | more |
the SDSPAGE profile of cream milk treated with actinidin reveals that both caseins, whey proteins and MFGF proteins are sharply digested by the enzyme |
Actinidia deliciosa |
? |
- |
? |
