EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.14.18.4 | eicosenoic acid + reduced acceptor + O2 |
- |
Physaria fendleri |
lesquerolic acid + acceptor + H2O |
- |
? |
| 1.14.18.4 | linoleic acid + ferrocytochrome P-450 + O2 |
- |
Claviceps purpurea |
linolenic acid + ferricytochrome P-450 + H2O |
- |
? |
| 1.14.18.4 | more |
biosynthesis of ricinoleic acid in Claviceps purpurea is catalyzed by the fungal desaturase-like hydroxylase |
Claviceps purpurea |
? |
- |
? |
| 1.14.18.4 | more |
in Lesquerella fendleri ricinoleic acid is efficiently elongated to lesquerolic acid in a reaction catalyzed by a condensing enzyme specific to hydroxy fatty acids |
Physaria lindheimeri |
? |
- |
? |
| 1.14.18.4 | more |
the enzyme acts predominantly as a hydroxylase, introducing hydroxyl groups at the 12-position of oleic acid and palmitoleic acid. It also shows DELTA12 desaturase activities on 16C and 18C monounsaturated fatty acids and, to a much lesser extent, omega3 desaturase activities on ricinoleic acid, overview |
Claviceps purpurea |
? |
- |
? |
| 1.14.18.4 | more |
the activitiy of oleoyl-12-hydroxylase seems unaffected by the fatty acid at the sn-1 position of 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine. 1-acyl-2-ricinoleoyl-sn-glycero-3-phosphocholine is formed mostly by the hydroxylation of 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine, not from the incorporation of ricinoleate into 1-acyl-2-ricinoleoyl-sn-glycero-3-phosphocholine, and 1-acyl-2-oleoyl-sn-glycero-3-phosphoethanolamine is less actively formed than 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine |
Ricinus communis |
? |
- |
? |
| 1.14.18.4 | oleate + ferrocytochrome P-450 + H+ + O2 |
- |
Claviceps purpurea |
12-hydroxy-oleate + ferricytochrome P-450 + H2O |
- |
? |
| 1.14.18.4 | oleate + ferrocytochrome P-450 + H+ + O2 |
the enzyme is primarily a fatty acid hydroxylase, rather than a bifunctional oleate 12-hydroxylase-desaturase, involved in ricinoleic acid biosynthesis, overview |
Physaria lindheimeri |
12-hydroxy-oleate + ferricytochrome P-450 + H2O |
- |
? |
| 1.14.18.4 | oleate + ferrocytochrome P-450 + H+ + O2 |
the enzyme is primarily a fatty acid hydroxylase rather than a bifunctional oleate 12-hydroxylase-desaturase, overview |
Physaria lindheimeri |
12-hydroxy-oleate + ferricytochrome P-450 + H2O |
- |
? |
| 1.14.18.4 | oleic acid + 2 ferrocytochrome b5 + O2 + 2 H+ |
- |
Claviceps purpurea |
ricinoleic acid + 2 ferricytochrome b5 + H2O |
- |
? |
