EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.15.1 | more |
putidaredoxin (Pdx) and putidaredoxin reductase (PdR) are expressed in Escherichia coli strain BL21 from a pMG211 plasmid as C-terminally His6-tagged proteins, and purified by nickel affinity chromatography and gel filtration |
Pseudomonas putida ATCC 12633 |
? |
- |
? |
1.14.15.1 | more |
catalytic turnover in P450cam requires the enzymes putidaredoxin (Pdx) and putidaredoxin reductase (Pdr), which mediate electron transfer from NADH to heme, the process is tightly coupled to substrate hydroxylation |
Pseudomonas putida ATCC 12633 |
? |
- |
? |
1.14.15.1 | norcamphor + O2 + reduced putidaredoxin |
- |
Pseudomonas putida |
? |
- |
? |
1.14.15.1 | norcamphor + reduced putidaredoxin + O2 |
- |
Pseudomonas putida |
? |
- |
? |
1.14.15.1 | norcamphor + reduced putidaredoxin + O2 |
- |
Pseudomonas putida |
? + oxidized putidaredoxin + H2O |
- |
? |
1.14.15.1 | peracetic acid + O2 + reduced putidaredoxin |
- |
Pseudomonas putida |
? |
- |
? |
1.14.15.1 | peracetic acid + O2 + reduced putidaredoxin |
reaction mechanism of substrate-free ferric cytochrome P450cam, via FeIV-O plus tyrosyl radical, overview |
Pseudomonas putida |
? |
- |
? |
1.14.15.1 | phenanthrene + putidaredoxin + O2 |
- |
Pseudomonas putida |
1-phenanthrol + 2-phenanthrol + 3-phenanthrol + 4-phenanthrol + oxidized putidaredoxin + H2O |
- |
? |
1.14.15.1 | pyrene + putidaredoxin + O2 |
- |
Pseudomonas putida |
1-pyrenol + 2-pyrenol + 1,6-pyrenequinone + 1,8-pyrenequinone + oxidized putidaredoxin + H2O |
- |
? |
1.14.15.1 | thiocamphor + reduced putidaredoxin + O2 |
- |
Pseudomonas putida |
? + oxidized putidaredoxin + H2O |
- |
? |