EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.13.160 | more |
reaction of different 4-substituted cyclohexanones with small to medium substituents at the C-4 position in nearly all cases give enantiopure lactones with an excellent yield |
Pseudomonas putida ATCC 17453 |
? |
- |
? |
1.14.13.160 | more |
no substrate: 2,5-diketocamphane. Less than 5% of the activity with 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA is displayed with 2-oxo-DELTA3-4,5,5-trimethylcyclo-pentenylacetyl-CoA, 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetyl-CoA (hydrolyzed), 2-oxo-DELTA3-4,5,5-trimethylcyclopentenylacetate-CoA, 2,5-diketocamphane |
Pseudomonas putida C1 / ATCC 17453 |
? |
- |
? |
1.14.13.160 | norcamphor + O2 + NADPH + H+ |
- |
Pseudomonas putida |
? + NADP+ + H2O |
- |
? |
1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ |
- |
Pseudomonas putida |
[(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O |
- |
? |
1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ |
- |
Pseudomonas putida |
[(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O |
mass spectral analysis of the methyl ester of the product confirmes the insertion of a single oxygen atom |
? |
1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ |
- |
Pseudomonas putida C1 / ATCC 17453 |
[(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O |
- |
? |
1.14.13.160 | [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ |
- |
Pseudomonas putida C1 / ATCC 17453 |
[(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O |
mass spectral analysis of the methyl ester of the product confirmes the insertion of a single oxygen atom |
? |