EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.4.22.14 | kiwellin + H2O |
in vitro treatment of purified kiwellin, an allergenic protein formerly isolated from green kiwi fruit, with the protease actinidin from green kiwi fruit originates KiTH, a 20 kDa protein with 100% identity with the C-terminal region of kiwellin, and kissper, a described pore-forming peptide |
Actinidia deliciosa |
KiTH + kissper |
- |
? |
3.4.22.14 | more |
actinidin compared with type II or IV collagenase isolated from intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90% |
Actinidia deliciosa |
? |
- |
? |
3.4.22.14 | more |
digestibility of kiwifruit allergens, actinidin (Act d 1) and thaumatin-like protein (Act d 2), is assessed using an in vitro digestion system that approximates physiological conditions with respect to the passage of food through the stomach into the duodenum: Act d 1 precipitates in simulated gastric fluid at pH 2 and digestion of the aggregated protein proceeds slowly. The residual precipitate redissolves completely in simulated duodenal fluid at pH 6.5 and is partially digested. Act d 1 and Act d 2 display nearly unchanged IgE binding abilities |
Actinidia deliciosa |
? |
- |
? |
3.4.22.14 | more |
the SDSPAGE profile of cream milk treated with actinidin reveals that both caseins, whey proteins and MFGF proteins are sharply digested by the enzyme |
Actinidia deliciosa |
? |
- |
? |
3.4.22.14 | N-alpha-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O |
- |
Actinidia deliciosa |
N-alpha-benzyloxycarbonyl-L-lysine + 4-nitrophenol |
- |
? |
3.4.22.14 | Nalpha-benzyloxycarbonyl-L-Lys 4-nitrophenyl ester + H2O |
- |
Actinidia deliciosa |
Nalpha-benzyloxycarbonyl-L-Lys + 4-nitrophenol |
- |
? |
3.4.22.14 | occludin + H2O |
- |
Actinidia deliciosa |
? |
- |
? |
3.4.22.14 | sheep IgG + H2O |
actinidin is ineffective on the heavy chain but has limited effects on the light chain |
Actinidia deliciosa |
? |
- |
? |
3.4.22.14 | casein + H2O |
efficiently hydrolized in acidic pHs |
Actinidia deliciosa cv. Hayward |
? |
- |
? |
3.4.22.14 | Collagen + H2O |
efficiently hydrolized at neutral or mild basic pH |
Actinidia deliciosa cv. Hayward |
? |
- |
? |