EC Number |
Storage Stability |
---|
6.3.2.3 | -18°C, stable for at least 1 year |
6.3.2.3 | -20°C, 20% loss of activity after 2 months |
6.3.2.3 | -20°C, purified enzyme, 30% glycerol, at least 2 years stable |
6.3.2.3 | -20°C, stable for weeks |
6.3.2.3 | -80°C, 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, can be stored for up to 6 months without significant loss of activity |
6.3.2.3 | -80°C, in 50 mM Tris-HCl (pH 7.4) containing100 mM NaCl, up to 6 months, without significant loss of activity |
6.3.2.3 | 4°C or frozen, in presence of glutathione and 5 mM DTT, stable for 1 month |
6.3.2.3 | 4°C, -20°C or -80°C, the isolated enzyme loses activity quickly (about 50% in 24 h), with or without glycerol |
6.3.2.3 | 4°C, when stored in 20 mM Tris-Cl and 1 mM EDTA, pH 8.6, in sterile cryogenic tubes at 4°C, the recombinant hGS dimer interface mutant enzymes lose activity in the 30 hours after purification. Both R221A and D24A lose activity in a biphasic manner within a few hours: D24A loses 30% of activity in 4 hours, while the activity of R221A decreases by 20% in 7.5 hours |
6.3.2.3 | stable for more than 2 months |