EC Number   |
Storage Stability |
|---|
    4.1.1.23 | -15°C, storage of the high molecular weight forms in presence of 0.15 mM oxipurinol prevents the spontaneous dissociation which occures when these forms are stored in 50 mM potassium phosphate, pH 7.4 |
| 4.1.1.23 | -20°C, 20% glycerol, retention of full activity for more than 2 years |
| 4.1.1.23 | -25°C, stable |
| 4.1.1.23 | -70°C, dilute buffer plus 2 mM dithiothreitol, purified UMP synthase activity retains orotidine-5'-phosphate decarboxylase activity for several months |
| 4.1.1.23 | 0-4°C, stable in 10 mM potassium phosphate buffer, pH 5.8-7.5, containing 5 mM 2-mercaptoethanol for several weeks |
| 4.1.1.23 | 0°C or -70°C, 5 mM potassium phosphate, pH 7.0, 2 mM dithiothreitol, rapid loss of orotidine-5'-phosphate decarboxylase activity of the orotidine-5'-phosphate decarboxylase domain of UMP synthase |
| 4.1.1.23 | 4°C, purified recombinant bifunctional enzyme, stability property of both domains in the bifunctional enzyme in the absence or presence of 0.5 mM PRPP and 0.5 mM UMP: half-life of the OPRT activity is about 3-4 days and 3 weeks, respectively. The OMPDC activity is much more stable with half-life of about 3-4 weeks. 90% of the bifunctional protein, stored for 4 weeks, remains as a bifunctional protein band in SDS-PAGE and image analyses |
| 4.1.1.23 | 4°C, stable for 1 week |
| 4.1.1.23 | 4°C, stable for 16 months |
