EC Number   |
Storage Stability |
|---|
    3.5.1.4 | -20°C 0.35 M potassium phosphate with 0.5 mM DTT, 10 micromol CaCl2 and 15% glycerol, several weeks without loss of activity |
| 3.5.1.4 | -30°C, 0.05 M Tris buffer, pH 7.2, 50% v/v glycerol, 1 mM 2-mercaptoethanol |
| 3.5.1.4 | 4°C, purified enzyme, in 50 mM phosphate buffer, more than 6 months without loss of activity |
| 3.5.1.4 | 4°C, the purified enzyme can be stored for longer than 6 months at in 50 mM phosphate buffer and 10% glycerol without any loss of activity |
| 3.5.1.4 | glycerol 2%, v/v, stabilizes the enzyme, after storing three months at -18°C, 77% of the activity of purified enzyme recovered |
| 3.5.1.4 | purified amidase stored in 0.05 M Tris buffer, pH 7.2, containing 1 mM mercaptoethanol and 50%, v/v, glycerol, at -30°C |
| 3.5.1.4 | room temperature, half-life about 2000 days with ammonium sulfate |
| 3.5.1.4 | stable to freezing |
| 3.5.1.4 | the enzyme may be stored at room temperature in the presence of 0.04% NaN3 for 1 year without any loss of activity |
| 3.5.1.4 | unstable even at 4°C |
