EC Number |
Storage Stability |
---|
3.5.1.15 | -20°C, frozen storage of undialyzed enzyme for a few days leads to a complete disappearance of activity following a single thaw |
3.5.1.15 | -70°C, kept for up to 4 weeks without loss of activity |
3.5.1.15 | 4°C, purified wild-type enzyme, expressed |
3.5.1.15 | the purified enzyme expressed in Pichia pastoris is significantly more stable than the Escherichia coli-expressed enzyme, losing less than 10% of its catalytic activity after 2 weeks when stored under conditions where the Escherichia coli-expressed enzyme becomes completely inactivated within 24 h, the Pichia pastoris-expressed enzyme shows sensitivity to oxidation over time and will precipitate if not kept under the proper reducing conditions |