EC Number   |
Storage Stability |
|---|
   3.1.25.1 | -12°C, in 12% glycerol, inactivates the enzyme |
| 3.1.25.1 | -20°C, addition of an equal value of glycerol |
| 3.1.25.1 | -20°C, addition of an equal value of glycerol, 1 ml aliquots |
| 3.1.25.1 | -20°C, in 50% glycerol, complete inactivation in 3-4 weeks |
| 3.1.25.1 | -70°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 0.01% Triton X-100, 50 mM KCl, 6 months, loss of 55% activity |
| 3.1.25.1 | 4°C, in 10 mM Tris-HCl buffer, pH 8.0, with 3% polyethylene glycol and 0.1 mM EDTA |
| 3.1.25.1 | 4°C, in 50 mM Tris-HCl, pH 7.5, containing 10% ethylene glycol or 3% polyethylene glycol, storage for several months |
| 3.1.25.1 | 4°C, stock enzyme-containing cell extracts |
| 3.1.25.1 | freezing, leads to inactivation |
| 3.1.25.1 | in liquid nitrogen, for minimal inactivation |
