EC Number |
Storage Stability |
---|
2.4.2.14 | -20°C or 4°C, cell-free extract, overnight, more than 50% loss of activity |
2.4.2.14 | -20°C, crude, more than 50% loss of activity overnight |
2.4.2.14 | 2-mercaptoethanol and phosphoribosyldiphosphate stabilize during storage at 4°C |
2.4.2.14 | 2-mercaptoethanol stabilizes during storage |
2.4.2.14 | 3°C, crude mutant enzyme extract, inactivation overnight, partially purified mutant enzyme: inactivation within 72 h, PMSF stabilizes |
2.4.2.14 | 3°C, crude or partially purified wild-type enzyme, at least 72 h stable |
2.4.2.14 | 4°C, 25% loss of activity per day |
2.4.2.14 | 4°C, 50 mM phosphate bufer, pH 7.4, 5 mM MgCl2, 60 mM 2-mercaptoethanol, at least 4 weeks, no loss of activity |
2.4.2.14 | 4°C, in phosphate buffer, pH 7.4, 60 mM 2-mercaptoethanol and phosphoribosyldiphosphate, at least 10 days |
2.4.2.14 | 4°C, partially purified preparation in 50 mM phosphate buffer, pH 7.4, 5 mM Mg2+ and 60 mM 2-mercaptoethanol, at least 4 weeks |