EC Number   |
Storage Stability |
|---|
    2.4.2.10 | -20°C, concentrated enzyme solution, at least 4 months |
| 2.4.2.10 | -20°C, pH 7.5-9.5, at least 6 months |
| 2.4.2.10 | -25°C, in 50% v/v glycerol, 0.1 mM DTT, about 35% loss of activity within 40 days |
| 2.4.2.10 | -60°C to-20°C, crude enzyme or ammonium sulfate preparation, 2 years |
| 2.4.2.10 | -76°C, at least 6 months |
| 2.4.2.10 | -80°C, stable up to 12 months, 5% glycerol, pH 8.0 |
| 2.4.2.10 | 4°C, 50 mM Tris-HCl, 2 mM EDTA, 2 mM mercaptoethanol, pH 6.4, several months, no apparent loss of activity |
| 2.4.2.10 | 4°C, gradual loss of activity at pH 7.5-9.5 |
| 2.4.2.10 | 4°C, purified recombinant bifunctional enzyme, stability property of both domains in the bifunctional enzyme in the absence or presence of 0.5 mM PRPP and 0.5 mM UMP: half-life of the OPRT activity is about 3-4 days and 3 weeks, respectively. The OMPDC activity is much more stable with half-life of about 3-4 weeks. 90% of the bifunctional protein, stored for 4 weeks, remains as a bifunctional protein band in SDS-PAGE and image analyses |
| 2.4.2.10 | 4°C, t1/2: 1.5 days |
