EC Number |
Storage Stability |
---|
1.14.15.1 | -196°C, no loss of activity of putidaredoxin reductase after repeated freeze-thaw cycles |
1.14.15.1 | -20°C, 50 mM Tris buffer, pH 7.4, 50% v/v glycerol |
1.14.15.1 | -80°C |
1.14.15.1 | -80°C, 50 mM potassium phosphate buffer, pH 7.4, 50 mM KCl, 1 mM (+)-camphor |
1.14.15.1 | 0-4°C, after 24-96 h, the resuspended pellet of 140000*g sedimentation of Pseudomonas sp. C5 loses hydroxylation capacity, restorable with NADPH and THF |
1.14.15.1 | 0°C, putidaredoxin slowly loses its prosthetic group, 2-mercaptoethanol retards apoprotein formation |
1.14.15.1 | 4°C, monomeric cytochrome P450cam can be stored at low protein concentrations for several days without appreciable accumulation of the dimer |