EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   7.4.2.5 | -999 |
- |
10 nM Pi/min/nM SecA in presence of Escherichia coli PE/anionic phospholipid membranes |
695878 |
| 7.4.2.5 | -999 |
- |
425 mol ATP/min/mol SecA in presence of 0.2 micromol preprotein translocation substrate proOmpA in urea |
697949 |
| 7.4.2.5 | -999 |
- |
circa 1.8 pMol Pi/pMol 6His-SecA2/min, measured in an assay containing 2.5 mM Mg2+ |
698615 |
| 7.4.2.5 | -999 |
- |
circa 7 pMol phosphate/pMol 6His-SecA/min measured in an assay containing 0.1 mM Mg2+ |
698615 |
| 7.4.2.5 | -999 |
- |
in ATPase translocation activity assay with preprotein translocation substrate proOmpA: circa 0.8 mM ATP/2 micromol SecA after 1 min, mutant SecA proteins with deletions of amino acids between 2-11 shows ATPase translocation activity around 0.25 mM ATP/2 micromol SecA after 1 min |
698635 |
| 7.4.2.5 | -999 |
- |
translocation ATPase activity: 0.9 nmol phosphate/microgramm SecA/min, mutants lacking 2 to 11 residues of the amino terminus of SecA shows defect translocation ATPase activities (below 0.1 nmol phosphate/microgramm/min) |
698596 |
| 7.4.2.5 | 0.00000022 |
- |
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP |
687663 |
| 7.4.2.5 | 0.00000022 |
- |
peptide transport rate, wild type, in the presence of 3 mM ATP and 1 mM orthovanadate |
687663 |
| 7.4.2.5 | 0.00000026 |
- |
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP and 500 microM competitor peptide RRYQKSTEL |
687663 |
| 7.4.2.5 | 0.00000026 |
- |
peptide transport rate, wild type, in the presence of 3 mM AMP |
687663 |
