EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    5.1.1.5 | -999 |
- |
enzyme is incubated in 1 ml of reaction mixture containing 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride and 10 mM L- or D-lysine at 30°C, Lyr activities in 4 week old wild type and transgenic tobacco plants are determined, the specific activity of Lyr in the transgenic T2 plants selected on L-lysine ranges from 0.77 to 1.06 mU/mg protein, although transgenic plants exhibit considerable variation in enzyme activities, no phenotypic dissimilarities associated with L-lysine selection are found, suggesting that the Lyr gene as a selectable marker could be effective over a range of expression levels |
706257 |
| 5.1.1.5 | 1.68 |
- |
substrate 10 mM D-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
701800 |
| 5.1.1.5 | 3.61 |
- |
substrate 10 mM L-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
701800 |
| 5.1.1.5 | 568 |
- |
purified recombinant His-tagged enzyme, substrate L-arginine, pH 8.0, 50°C |
728711 |
| 5.1.1.5 | 887 |
- |
purified recombinant His-tagged mutant S394Y, substrate L-lysine, pH 8.0, 50°C |
728711 |
| 5.1.1.5 | 980 |
- |
purified recombinant His-tagged mutant S394C, substrate L-arginine, pH 8.0, 50°C |
728711 |
| 5.1.1.5 | 1043 |
- |
purified recombinant His-tagged mutant S394T, substrate L-arginine, pH 8.0, 50°C |
728711 |
| 5.1.1.5 | 1185 |
- |
purified recombinant His-tagged mutant S394Y, substrate L-arginine, pH 8.0, 50°C |
728711 |
| 5.1.1.5 | 1200 |
- |
purified recombinant His-tagged mutant S394N, substrate L-arginine, pH 8.0, 50°C |
728711 |
| 5.1.1.5 | 1633 |
- |
purified recombinant His-tagged mutant S394N, substrate L-lysine, pH 8.0, 50°C |
728711 |
