EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    5.1.1.13 | -999 |
- |
the pituitary gland is the tissue which contains the highest enzymatic activity, with 20.0 EU/mg protein, followed by: testis; brain, liver, kidney and serum, 1 enzyme unit (EU) is defined as the amount of the enzyme capable of generating 1 nmol of D-Asp in 60 min of incubation at 37°C and in the assay conditions (0.1 M Tris-HCl,10 mM EDTA, 0.1 M sodium L-aspartate) |
706688 |
| 5.1.1.13 | 4.75 |
- |
- |
661647 |
| 5.1.1.13 | 7.39 |
- |
for L-aspartate |
650892 |
| 5.1.1.13 | 19.5 |
- |
crude recombinant enzyme in Escherichia coli cell extract, coupled assay method, pH 8.3, 37°C |
748452 |
| 5.1.1.13 | 19.9 |
- |
- |
2078 |
| 5.1.1.13 | 22.6 |
- |
for D-aspartate |
650892 |
| 5.1.1.13 | 26.8 |
- |
- |
2077 |
| 5.1.1.13 | 460 |
- |
Vmax of recombinant enzyme, activity is assayed in 500 nl of pyrydoxal 5'-phosphate (100 nM), 0.49 ml of recombinant enzyme (0.5 mg/ml), and 0.01 mL of 1 mM L-aspartate at 37°C for 1 h in 10 mM Tris (pH 7.5) |
706558 |
| 5.1.1.13 | 1160 |
- |
70°C, pH not specified in the publication |
726850 |
| 5.1.1.13 | 1550 |
- |
crude recombinant, pH 8.0, 90°C |
747721 |
