EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   4.6.1.18 | -999 |
- |
enzymatic activities of the various RNase A monomeric and zero-length or domain-swapped oligomeric species on single-stranded yeast or double-stranded poly(A)-poly(U) RNA, overview. The dimers show higher activity compared to the monomners |
717298 |
| 4.6.1.18 | -999 |
- |
for assay conditions ribonuclease A solution prepared by dissolving 8 mg ribonuclease A in 4 ml 0.1 M NaHCO3 at pH 9.5 following by pre-cooling to 3°C |
678356 |
| 4.6.1.18 | -999 |
- |
isoenzyme D-I shows values of 88 U/mg, 382 U/mg and 6.2 U/mg with yeast RNA, poly(U) and poly(A)poly(U) as substrates, respectively. D-II isoenzyme shows values of 85 U/mg, 387 U/mg and 2.5 U/mg with yeast RNA, poly(U) and poly(A)poly(U) as substrates, respectively |
655538 |
| 4.6.1.18 | -999 |
- |
type I isoform, 100% specific activity relative to commercial RNase A. Type II isoform, 53% specific activity relative to commercial enzyme |
654993 |
| 4.6.1.18 | 0.017 |
- |
pH 5.0, 37°C |
654814 |
| 4.6.1.18 | 1.14 |
- |
substrate poly(A)poly(U), dimethyl suberimidate-treated, monomeric RNase A, pH 7.0, 25°C |
678502 |
| 4.6.1.18 | 1.55 |
- |
substrate poly(A)poly(U), native, monomeric RNase A, pH 7.0, 25°C |
678502 |
| 4.6.1.18 | 2.22 |
- |
substrate poly(A)poly(U), cross-linked RNase A dimer, pH 7.0, 25°C |
678502 |
| 4.6.1.18 | 3 |
- |
enzyme monomer after lyophilization |
681511 |
| 4.6.1.18 | 3.52 |
- |
pH 7.0, 65°C |
677904 |
