EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
4.1.3.3 | -999 |
- |
- |
33314, 33316, 33319, 33321, 33325 |
4.1.3.3 | -999 |
- |
after a 36 h incubation, the concentrations of N-acetyl-D-neuraminic acid from the reactions utilizing 0.4, 0.8, 1.2, 1.6, 2.0, and 2.2 M pyruvate as the carbon source are 7.5 (3.75%), 26.9 (3.45%), 43.9 (21.95%), 56.0 (28%), and 61.9 mM (30.95%), respectively. When using 2.2 M pyruvate, in the first 6 h, the concentration of N-acetyl-D-neuraminic acid reaches 29.5 mM (which is about half of the maximal concentration), and the productivity of N-acetyl-D-neuraminic acid is 1.6 g/l/h, the concentration of the intermediate N-acetyl-D-mannosamine also increases gradually from 0 to 64 mM during the process |
701881 |
4.1.3.3 | -999 |
- |
cell density of Escherichia coli BL21 (DE3) carrying the pNA1 plasmid is 37 (OD600) after fermentation for 15 h, and the highest activity for N-acetyl-D-neuraminic acid lyase is 8.472 U/ml after fermentation for 9 h, enzyme activity is assayed in reaction solution (20 mM Neu5Ac in 50 mM potassium phosphate buffer, pH 7.5) |
701855 |
4.1.3.3 | 0.015 |
- |
N-acetyl-D-mannosamine + pyruvate, 37°C |
33337 |
4.1.3.3 | 0.0693 |
- |
immobilized recombinant enzyme, the activity yield of immobilized enzyme is approximately 10% of the free enzyme |
701855 |
4.1.3.3 | 0.14 |
- |
crude cell extract, at 37°C |
678004 |
4.1.3.3 | 0.42 |
1.5 |
- |
650651 |
4.1.3.3 | 1.2 |
2.2 |
N-acetylneuraminic acid, 25°C |
33336 |
4.1.3.3 | 1.5 |
- |
- |
651432 |
4.1.3.3 | 2.45 |
- |
crude extract, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C |
701855 |