EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
4.1.1.47 | 0.06 |
- |
mutant enzyme L478A, in 50 mM KH2PO4 (pH 7.7), 0.06 M KCl, 0.1 mM thiamin diphosphate, 5 mM MgCl2, at 37°C |
727003 |
4.1.1.47 | 0.2 |
- |
mutant enzyme V51D, in 50 mM KH2PO4 (pH 7.7), 0.06 M KCl, 0.1 mM thiamin diphosphate, 5 mM MgCl2, at 37°C |
727003 |
4.1.1.47 | 0.2 |
- |
mutant V51D catalyzes the formation of the product nearly two orders of magnitude more slowly than the wild-type enzyme |
705846 |
4.1.1.47 | 0.68 |
- |
mutant enzyme I393A, in 50 mM KH2PO4 (pH 7.7), 0.06 M KCl, 0.1 mM thiamin diphosphate, 5 mM MgCl2, at 37°C |
727003 |
4.1.1.47 | 0.84 |
- |
mutant enzyme I479V, in 50 mM KH2PO4 (pH 7.7), 0.06 M KCl, 0.1 mM thiamin diphosphate, 5 mM MgCl2, at 37°C |
727003 |
4.1.1.47 | 0.93 |
- |
mutant enzyme I393V, in 50 mM KH2PO4 (pH 7.7), 0.06 M KCl, 0.1 mM thiamin diphosphate, 5 mM MgCl2, at 37°C |
727003 |
4.1.1.47 | 2.7 |
- |
mutant V51E is about seven times slower than the wild-type enzyme |
705846 |
4.1.1.47 | 17.1 |
- |
mutant V51S is nearly as active as the wild-type enzyme |
705846 |
4.1.1.47 | 17.5 |
- |
wild type enzyme, in 50 mM KH2PO4 (pH 7.7), 0.06 M KCl, 0.1 mM thiamin diphosphate, 5 mM MgCl2, at 37°C |
727003 |
4.1.1.47 | 17.5 |
- |
wild-type enzyme |
705846 |