EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    3.2.2.9 | -999 |
- |
amide proton exchange rates of isozyme AtMTAN1 |
709718 |
| 3.2.2.9 | -999 |
- |
enzyme-coupled continuous spectrophotometric assay to quantitatively characterize S-adenosyl-L-methionine (AdoMet/SAM)-dependent methyltransferase activity. In this assay, S-adenosyl-L-homocysteine, the transmethylation product of AdoMet-dependent methyltransferases, is hydrolyzed to S-ribosylhomocysteine and adenine by recombinant S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase. Subsequently, adenine generated from AdoHcy is further hydrolyzed to hypoxanthine and ammonia by recombinant adenine deaminase |
677517 |
| 3.2.2.9 | 0.0152 |
- |
crude extract, with S-adenosyl-L-homocysteine as substrate, in 50 mM potassium phosphate buffer, pH 7.0, at 37°C |
171854 |
| 3.2.2.9 | 0.9 |
- |
using S-adenosyl-L-homocysteine as substrate, at pH 6.0 and 22°C |
681477 |
| 3.2.2.9 | 3.1 |
- |
Bgp protein, using S-adenosyl-L-homocysteine as substrate, pH 5.0 |
698517 |
| 3.2.2.9 | 3.6 |
- |
- |
171852 |
| 3.2.2.9 | 3.7 |
- |
Pfs protein, using S-adenosyl-L-homocysteine as substrate, pH 5.0 |
698517 |
| 3.2.2.9 | 3.75 |
- |
substrate S-adenosyl-L-homocysteine |
171850 |
| 3.2.2.9 | 9.93 |
- |
- |
171851 |
| 3.2.2.9 | 156 |
- |
after 9810fold purification, with S-adenosyl-L-homocysteine as substrate, in 50 mM potassium phosphate buffer, pH 7.0, at 37°C |
171854 |
