EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.2.1.81 | 0.04 |
- |
culture supernatant, pH 7.0, 30°C |
714561 |
3.2.1.81 | 0.1 |
- |
AgaB, 1 ml of 0.125% melted agarose, 44°C, pH 7.5 |
667490 |
3.2.1.81 | 0.16 |
- |
AgaAc, 1 ml of 0.125% melted agarose, 44°C, pH 7.5 |
667490 |
3.2.1.81 | 0.7 |
- |
crude enzyme from culture fluid, in 50 mM phosphate buffer, pH 7.0, 35°C |
715875 |
3.2.1.81 | 0.872 |
- |
cell lysate |
695736 |
3.2.1.81 | 0.9 |
- |
cell-free extract, at pH 7.0 and 35°C |
732321 |
3.2.1.81 | 1.2 |
- |
wild-type |
678815 |
3.2.1.81 | 3 |
- |
cell-free medium, at pH 7.0 and 35°C |
667288 |
3.2.1.81 | 4.13 |
- |
concentrated agarase solution |
698527 |
3.2.1.81 | 4.22 |
- |
after 103.5fold purification, pH 7.0, 30°C |
714561 |