EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
2.7.9.1 | -999 |
- |
- |
645419, 645420, 645425, 645429, 645430, 645431, 645444 |
2.7.9.1 | -999 |
- |
activities measured using a firefly luciferase-luciferin reaction |
642198 |
2.7.9.1 | -999 |
- |
activity and amounts of PPDK downregulated during seed development in rice, tissue-specificy, immunoblot analysis of different seed fractions of rice, SDS-PAGE, spectrophotometric assay of PPDK acitivity, kinetics |
676721 |
2.7.9.1 | -999 |
- |
enzyme activity per g of fresh tissue |
645435 |
2.7.9.1 | -999 |
- |
relationship between developmental stage and activity |
645435 |
2.7.9.1 | -999 |
- |
specific activity of PPDK in plants transformed with the construct showing 17 amino acid substitutions inversely correlates with amount of enzyme in leaves, PPDK activity becomes constant when the amount is above 5 mg/g fresh weight, enzyme contents estimated by Western blot, spectrometric assay at 25°C, two transformants retained 70% of activity after 180 min indicating a comparable cold-tolerance like Flaveria brownii plants, further effect of cold-tolerant PPDK on photosynthetic rate estimated by measurement of CO2 uptake at leaf temperatures of 30°C, 20°C, 13°C, and 8°C, increase by 23% at 8°C shown, no effects at higher temperatures |
675905 |
2.7.9.1 | -999 |
- |
the maximal specific activity of the catabolic reaction is approximately 2fold higher if compared to the anabolic reaction, indicated by lower Km-values for reaction compounds of the catabolic reaction, kinetics at 55°C and 70°C shown, neither inhibition nor activation of anabolic PPDK activity observed |
676065 |
2.7.9.1 | 0.0006 |
- |
leaf enzyme extract, pH and temperature not specified in the publication |
738921 |
2.7.9.1 | 0.005 |
- |
native enzyme in purified glycosomes, pH 8.3, temperature not specified in the publication |
738186 |
2.7.9.1 | 0.016 |
- |
purified glycosomes, pH 7.2, 28°C |
738185 |