EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    2.5.1.55 | 0.01 |
- |
N23C/C246S/D247E mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
| 2.5.1.55 | 0.01 |
- |
N23C/C246S/D247E/P249A mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
| 2.5.1.55 | 0.038 |
- |
- |
637393, 637394 |
| 2.5.1.55 | 0.06 |
- |
N23C/C246S/P249A mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
| 2.5.1.55 | 0.19 |
- |
N23C/C246S mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
| 2.5.1.55 | 0.3 |
- |
N23C/C247E mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
| 2.5.1.55 | 0.35 |
- |
N23C/C247E/P249A mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
| 2.5.1.55 | 0.6 |
- |
crude cell lysate, at 25°C and pH 7.5 |
685559 |
| 2.5.1.55 | 0.76 |
- |
N23C/P249A mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
| 2.5.1.55 | 1.9 |
- |
N23C mutant control, 200 microM phosphoenolpyruvate, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
