EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   1.14.20.1 | -999 |
- |
- |
440376, 440378 |
| 1.14.20.1 | -999 |
- |
160% relative activity with 10 mM substrate penicillin G compared to the activity detected with 1 mM penicillin G |
671443 |
| 1.14.20.1 | -999 |
- |
activities of wild-type enzyme in ring-expansion and hydroxylation, cosubstrate and substrate conversion rate |
659322 |
| 1.14.20.1 | -999 |
- |
activity of recombinant wild-type and mutant enzymes with substrate penicillin G and penicillin N |
657583 |
| 1.14.20.1 | -999 |
- |
activity with different substrates and assay methods of recombinant wild-type and mutant enzymes, overview |
440371 |
| 1.14.20.1 | -999 |
- |
quaternary mutant (C155Y/Y184H/V275I/C281Y) with enhanced activity and without substrate inhibition |
699366 |
| 1.14.20.1 | -999 |
- |
relative activities of wild-type and R308 mutants with penicillin variants and analogue substrates, overview |
714589 |
| 1.14.20.1 | -999 |
- |
the activity of scDAOCS very much depends on the stability of the enzyme maintained by equilibrium in a pool of monomeric and trimeric forms of scDAOCS which can be disrupted by introduction of a relatively small His-tag at the N-terminus of the enzyme |
699366 |
| 1.14.20.1 | -999 |
- |
to achieve optimum conditions for the conversion of penicillin to a cephalosporin, a reaction mixture containing 50 mM Tris-HCl (pH 7.4), 10 mM KCl, 10 mM MgSO4, 0.6 mM ascorbic acid, 0.8 mM ATP, 0.04 mM FeSO4, 0.6 mM 2-oxoglutarate, and 0.28 mM penicillin N is used for detection of the ring-expansion activity of DAOCS from Acremonium chrysogenum, DAOCS is unable to convert penicillin G and other penicillin N analogs under this optimum conditions described for penicillin N |
699366 |
| 1.14.20.1 | 0.013 |
- |
recombinant enzyme, substrate amoxicillin |
440374 |
