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Results 1 - 10 of 10
EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Reference
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363-999 - a method is described which enables one to assay simultaneously the NAD+- and NADP+-linked reactions of dehydrogenases which can utilize both coenzymes. The method is based on the fact that the thionicotinamide analogs of NADH and NADPH absorb light maximally at 400 nm, a wavelength sufficiently far removed from the absorbance maximum of NADH and NADPH to permit measurements of the simultaneous reduction of NAD+ (or NADP+) and the thionicotinamide analog of NADP+ (or NAD+). Application of the method to glucosed 6-phosphate dehydrogenase from Leuconostoc mesenteroides reveals differential effects of glucose 6-phosphate concentration on the NAD+- and NADP+-linked reactions catalyzed by this enzyme which can not be detected by conventional assay procedures and which may have regulatory significance 722581
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363-999 - the His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme 742533
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3630.006 - enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NADP+ 742533
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3630.006 - with NADP+ as cofactor, at pH 8.0 and 30°C 740461
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3630.008 - recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NAD+ 742533
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3630.025 - enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NAD+ 742533
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3630.025 - with NAD+ as cofactor, at pH 8.0 and 30°C 740461
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3630.103 - recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NADP+ 742533
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363145.7 - pH 8.0, 30°C, NADP+ 762314
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363715 - 25°C, pH 7.6, wild-type enzyme 723718
Results 1 - 10 of 10