EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   1.1.1.300 | 0.012 |
- |
mutant enzyme C300S, with retinal and NADPH as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.0386 |
- |
mutant enzyme C300S, with retinal and NAD+ as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.068 |
- |
mutant enzyme C300A, with retinal and NADPH as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.08 |
- |
mutant enzyme E266A, with retinal and NADPH as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.136 |
- |
wild type enzyme, with retinal and NADPH as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.26 |
- |
mutant enzyme E457V, with retinal and NADPH as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.408 |
- |
mutant enzyme E194S, with retinal and NADPH as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.461 |
- |
wild type enzyme, with retinal and NAD+ as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.493 |
- |
mutant enzyme E194S, with retinal and NAD+ as substrates, at pH 7.0 and 37°C |
739913 |
| 1.1.1.300 | 0.63 |
- |
eluate after purification, the specific activity of the purified enzyme with all-trans-retinal as substrate is 13fold higher than that of the microsomal preparation of wild-type |
654687 |
