EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    1.1.1.132 | -999 |
- |
- |
667316 |
| 1.1.1.132 | -999 |
- |
higher enzyme activities after IPTG induction only for pPT3 containing strains, low GDP-mannuronate production with pMMB66EH only detectable in mucoid strains, higher with pPT3 with increase in cells of mucoid and non-mucoid strains with IPTG, alginate formation occurs only in mucoid strains without significant increase with IPTG, later enzyme of alginate biosynthesis suggested to become rate-limiting |
285842 |
| 1.1.1.132 | -999 |
- |
increase of activity has only limited effect on the stimulation of alginate synthesis, existance of other rate-limiting enzymatic step suggested |
285837 |
| 1.1.1.132 | -999 |
- |
presence of 1 mM GDP-mannose, GDP-glucose, GMP, GDP, GTP or cGMP retards tryptic digest of enzyme and protects subsequent loss of enzyme activity, amino-terminal domain contains substrate and cofactor binding sites, carboxy-terminal domain is essential for catalytic activity |
285840 |
| 1.1.1.132 | 0.00015 |
- |
+/-0.00001, S17-1/pPT4, with IPTG |
285838 |
| 1.1.1.132 | 0.00017 |
- |
+/-0.00002, S17-1/pMMB66EH and S17-1/pPT4 without IPTG |
285838 |
| 1.1.1.132 | 0.00022 |
- |
+/-0.00001, S17-1/pMMB66EH, with IPTG |
285838 |
| 1.1.1.132 | 0.00034 |
- |
+/-0.00006, S17-1/pPT3, without IPTG |
285838 |
| 1.1.1.132 | 0.0004 |
- |
+/-0.00008, PAO1/pMMB66EH, mid-exponential, without IPTG |
285842 |
| 1.1.1.132 | 0.00056 |
- |
+/-0.00024, PAO1/pMMB66EH, early-stationary, with IPTG |
285842 |
