EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.4.22.53 | -999 |
- |
- |
643968, 643980, 643983, 644027, 644028, 644029, 644030, 644032, 644046 |
3.4.22.53 | -999 |
- |
initial activity as relative fluorescence units per s: 1.3 for wild type calpain 2, 0.84 for mutant G423R, 0.19 for mutant R628Q, 0.36 for mutant T344M, 0.7 for mutant D346E, 1.0 for mutant D362K |
696327 |
3.4.22.53 | -999 |
- |
endoplasmic reticulum membrane m-calpain shows 170 units/mg specific activity after 323fold purification, endoplasmic reticulum lumen m-calpain shows 324 units/mg specific activity after 463fold purification one unit is defined as the amount of the enzyme that causes a change of 1.0 in A750 for the trichloroacetic acid-soluble products after incubation of the enzyme in the assay mixture |
708960 |