EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.4.19.1 | -999 |
- |
- |
95253, 95254, 95267 |
3.4.19.1 | -999 |
- |
the specific activity of the enzyme increases 7–14fold with heat treatment, suggesting the modification of the structure of the enzyme for optimal hydrolytic activity by heating |
745261 |
3.4.19.1 | 0.0007 |
- |
cellular extract, using N-acetyl-L-Leu-4-nitroanilide as substrate, at pH 7.5 and 90°C |
720867 |
3.4.19.1 | 0.1 |
- |
pH 7.5, 80°C |
720431 |
3.4.19.1 | 0.124 |
- |
recombinant enzyme, after the induction with IPTG, at 8 h of culture in LB medium |
687846 |
3.4.19.1 | 0.172 |
- |
recombinant enzyme, after the induction with IPTG, at 18 h of culture in TB medium |
687846 |
3.4.19.1 | 1.9 |
- |
- |
651807 |
3.4.19.1 | 1.9 |
- |
substrate: acetyl-Tyr-4-nitroanilide, pH 5.4, 85°C |
745261 |
3.4.19.1 | 4.8 |
- |
after 6857fold purification, using N-acetyl-L-Leu-4-nitroanilide as substrate, at pH 7.5 and 90°C |
720867 |
3.4.19.1 | 4.8 |
- |
pH 7.5, 90°C |
720867 |