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Display the word mapDisplay the reaction diagram Show all sequences 7.4.2.6OppA and OppBCDF are reconstituted into giant unilamellar vesicles, overview 685623
Display the word mapDisplay the reaction diagram Show all sequences 7.4.2.6solubilization and refolding of recombinant insoluble His-tagged OppA expressed in Escherichia coli strain BL21(DE3) by dilution with 100fold volumes of refolding buffer, PBS, pH 7.4, containing 1 M urea, 10% glycerol, 0.005% Tween 20, 0.5 mM PMSF and 5 mM DTT, and maintainance under vigorous agitation at 4°C for 8 h 686956
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