EC Number   |
Reference |
|---|
    7.1.2.2 | denaturation of recombinant the cyano-epsilon, CF1-epsilon and the chimeric epsilon subunits, expressed from Escherichia coli strain BL21(DE3) as soluble proteins, using 8 M urea, and subsequent refolding |
711138 |
| 7.1.2.2 | F1 is reconstituted by using 3:3:1 molar ratios of recombinant alpha-, beta-, and gamma-subunits with a total protein concentration of 0.1 mg/ml, in the reconstitution dissolving buffer containing 10 mM Tris/succinate, pH 6.0, 0.2 mM 2-mercaptoethanol, 10% glycerol and 1% CHAPS, followed by dialysis against the reconstitution dialysis buffer containing 50 mM Tris/succinate, pH 6.0, 0.05 mM deferoxamine mesylate, 5 mM ATP, 2 mM MgCl2, 0.2 mM 2-mercaptoethanol, 10% glycerol and 1% CHAPS under constant stirring at room temperature |
711132 |
| 7.1.2.2 | purified native enzyme is reconstituted into liposomes |
685366 |
| 7.1.2.2 | purified native H+ FoF1-ATP synthase complex after purification |
696051 |
| 7.1.2.2 | reconstitution of purified recombinant mutant EFoF1 into liposomes using L-alpha-phosphatidylcholine from soybean suspended in 10 mM HEPES-NaOH, 5 mM MgSO4, and 1 mM KCl, pH 7.5 |
712371 |
| 7.1.2.2 | reconstitution of the purified enzyme in proteoliposomes |
711328 |
| 7.1.2.2 | stripping of F1 and Fo from recombinant membrane fragments, and functional purified FoF1s reconstitutions in proteoliposomes, suspended in 10 mM HEPES/NaOH, pH 7.5, 0.25 M sucrose, and 5 mM MgSO4, at 25°C |
711145 |
