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Renatured (Commentary)
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4.1.3.42
exposure to phosphoric acid at pH 1.6 for 10 min at 4°C causes 95% or greater inactivation. The inactivation process is characterized by an alteration of secondary and tertiary structure as well as an aggregation to a dimer of the native molecule. Reactivation of enzyme activity to 60-80% of the original level is seen within 20 min at pH 6 to 8
721748
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