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EC Number Renatured (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17construction of an in vitro reaction system in which the enzyme is membrane embedded and not solubilized by detergent, two inverted membrane vesicles or proteoliposomes, one bearing the enzyme, the other bearing the substrate protein, are fused, reconstituted enzyme is accessible to proteinase K because the ATPase and protease are exposed outside the vesicles 652391
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17recombinant enzyme, solubilization by N-lauylsarcosine, refolding in presence of ATP, Mg2+ and Zn2+, and purification to near homogeneity, the refolded enzyme has properties similar to the overexpressed solubilized one 650184
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