EC Number   |
Reference |
|---|
   3.4.22.65 | ProDer p 1, refolding after solubilization in urea |
698444 |
| 3.4.22.65 | recombinant His-tagged fusion proteins encompassing Der p 2 with either mature or proDer p 1 from Escherichia coli inclusion bodies with purification via denaturating nickel affinity chromatography |
717665 |
| 3.4.22.65 | recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea |
731895 |
| 3.4.22.65 | recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea |
731895 |
| 3.4.22.65 | recombinant proenzyme pro-Der f1 from Escherichia coli inclusion bodies, solubilization/degenration by 6 M urea, and refolding in refolding buffer containing 20 mM Tris.Cl pH 8.6, 1% glycerol, 1 mM EDTA, as well as 3 mM reduced glutathione, and 0.3 mM oxidized glutathione at a final concentration of 0.05 mg/ml, followed by gel filtration, method optimization, overview |
732865 |
